The use of clade-specific PCR assays to identify novel nitrilase genes from environmental isolates

Dooley-Cullinane, Tríona Marie and O'Reilly, Catherine and Aslam, Bilal and Weiner, David P. and O'Neill, David and Owens, Erica and O'Meara, Denise and Coffey, Lee (2019) The use of clade-specific PCR assays to identify novel nitrilase genes from environmental isolates. MicrobiologyOpen, 8 (4). ISSN 2045-8827

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Abstract

Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio-pharmaceuticals and industrial applications facilitates the move towards green chemistry. The body of research presented describes a novel clade-specific touchdown PCR protocol for the detection of novel nitrilase genes. The presented study identified partial sequences of 15 novel nitrilase genes across 7 genera, with partial DNA sequence homology (%) displayed across an additional 16 genera. This research will prove valuable in the screening of microorganisms for the identification of novel clade-specific nitrilase genes, with predicted enantioselective profiles as determined by their clade characterizations.

Item Type:	Article
Additional Information:	Publisher Copyright: © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
Uncontrolled Keywords:	/dk/atira/pure/subjectarea/asjc/2400/2404
Departments or Groups:
Depositing User:	Admin SSL
Date Deposited:	19 Oct 2022 23:02
Last Modified:	07 Jun 2023 18:41
URI:	http://repository-testing.wit.ie/id/eprint/3691

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