Development of a silica monolith modified with Fe3O4 nano-Particles in centrifugal spin column format for the extraction of phosphorylated compounds

Alwy, Ali and Clarke, Sarah P. and Brougham, Dermot F. and Twamley, Brendan and Paull, Brett and White, Blánaid and Connolly, Damian (2015) Development of a silica monolith modified with Fe3O4 nano-Particles in centrifugal spin column format for the extraction of phosphorylated compounds. Journal of Separation Science, 38 (2). pp. 283-290. ISSN 1615-9306

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Abstract

In this study, citrate-stabilised iron oxide nano-particles (~16 nm) have been immobilised on commercial silica monolithic centrifugal spin columns (MonoSpin) for the extraction of phosphorylated compounds. Two alternative strategies were adopted involving either direct electrostatic attachment to an aminated MonoSpin (single-layer method) in the first instance, or the use of a layer-by-layer method with poly(diallyldimethylammonium) chloride. Fieldemission scanning electron spectroscopy and energy-dispersive X-ray spectroscopy was used for confirming notably higher coverage of nano-particles using the layer-by-layer method (2.49 ± 0.53 wt%) compared with the single-layer method (0.43 ± 0.30 wt%). The modified monolith was used for the selective separation/extraction of adenosine monophosphate, adenosine diphosphate and adenosine triphosphate with elution using a phosphate buffer. A reversed-phase liquid chromatographic assay was used for confirming that adenosine, as a non-phosphorylated control was not retained on the modifiedMonoSpin devices, whereas recovery of 80% for adenosine monophosphate, 86% for adenosine diphosphate and 82% for adenosine triphosphate was achieved.

Item Type:	Article
Additional Information:	Publisher Copyright: © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Uncontrolled Keywords:	/dk/atira/pure/subjectarea/asjc/1600/1602
Departments or Groups:
Depositing User:	Admin SSL
Date Deposited:	19 Oct 2022 23:03
Last Modified:	22 Jun 2023 19:35
URI:	http://repository-testing.wit.ie/id/eprint/3766

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