Effect of seaweed supplementation on tocopherol concentrations in bovine milk using dispersive liquid-liquid microextraction

Quigley, Andrew and Walsh, Siobhán W. and Hayes, Eva and Connolly, Damian and Cummins, Wayne (2018) Effect of seaweed supplementation on tocopherol concentrations in bovine milk using dispersive liquid-liquid microextraction. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1092. pp. 152-157. ISSN 1570-0232

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Abstract

A dispersive liquid-liquid microextraction (DLLME) method, combined with HPLC-UV detection, was developed for the extraction and preconcentration of δ-tocopherol from bovine milk. This method was used to study the effect of supplementing cow feed with the seaweed Ascophyllum nodosum on vitamin content in milk. The optimal experimental conditions were determined: 200 μL of chloroform (extraction solvent), 1.0 mL of ethanol (dispersive solvent), 5 mL of water (aqueous phase). Under these optimal conditions the DLLME method provided linearity in the range 0.01 μg/mL to 8 μg/mL with R2 values of 0.998. Limit of detection (LOD) was 0.01 μg/mL, while the enrichment factor was 89. Cow feed that was supplemented with Ascophyllum nodosum was shown to increase δ-tocopherol levels from 3.82 μg/mL to 5.96 μg/mL.

Item Type: Article
Additional Information: Funding Information: The authors would like to thank the Irish Research Council [grant number GOIPG/2015/3681 ] for financial support of this work. Publisher Copyright: © 2018
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1600/1602
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Depositing User: Admin SSL
Date Deposited: 19 Oct 2022 23:04
Last Modified: 07 Jun 2023 18:42
URI: http://repository-testing.wit.ie/id/eprint/3872

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