Nisin in combination with cinnamaldehyde and EDTA to control growth of Escherichia coli strains of swine origin

Field, Des and Baghou, Inès and Rea, Mary C. and Gardiner, Gillian E. and Ross, R. Paul and Hill, Colin (2017) Nisin in combination with cinnamaldehyde and EDTA to control growth of Escherichia coli strains of swine origin. Antibiotics, 6 (4). ISSN 2079-6382

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Abstract

Post-weaning diarrhoea (PWD) due to enterotoxigenic Escherichia coli (ETEC) is an economically important disease in pig production worldwide. Although antibiotics have contributed significantly to mitigate the economic losses caused by PWD, there is major concern over the increased incidence of antimicrobial resistance among bacteria isolated from pigs. Consequently, suitable alternatives that are safe and effective are urgently required. Many naturally occurring compounds, including the antimicrobial peptide nisin and a number of plant essential oils, have been widely studied and are reported to be effective as antimicrobial agents against pathogenic microorganisms. Here, we evaluate the potential of nisin in combination with the essential oil cinnamaldehyde and ethylenediaminetetraacetic acid (EDTA) to control the growth of E. coli strains of swine origin including two characterized as ETEC. The results reveal that the use of nisin (10 µM) with low concentrations of trans-cinnamaldehyde (125 µg/mL) and EDTA (0.25–2%) resulted in extended lag phases of growth compared to when either antimicrobial is used alone. Further analysis through kill curves revealed that an approximate 1-log reduction in E. coli cell counts was observed against the majority of targets tested following 3 h incubation. These results highlight the potential benefits of combining the natural antimicrobial nisin with trans-cinnamaldehyde and EDTA as a new approach for the inhibition of E. coli strains of swine origin.

Item Type:	Article
Additional Information:	Funding Information: Acknowledgments: D.F., M.C.R., C.H. and R.R. are supported by the Irish Government under the National Development Plan, through SFI Investigator awards to C.H. and R.R. (10/IN.1/B3027), and the APC Microbiome Institute under Grant Number SFI/12/RC/2273. We thank Paula O’Connor for mass spectrometry analysis. We also thank Prof. Nola Leonard at the University College Dublin for providing E. coli isolates and the Agri-Food and BioSciences Institute (AFBI), Stormont, Northern Ireland, for providing ETEC strains. Publisher Copyright: © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
Uncontrolled Keywords:	/dk/atira/pure/subjectarea/asjc/2400/2404
Departments or Groups:
Depositing User:	Admin SSL
Date Deposited:	19 Oct 2022 23:04
Last Modified:	09 Aug 2023 22:05
URI:	http://repository-testing.wit.ie/id/eprint/3912

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