Quantification of zeaxanthin stereoisomers and lutein in trout flesh using chiral high-performance liquid chromatography-diode array detection

Prado-Cabrero, Alfonso and Beatty, Stephen and Stack, Jim and Howard, Alan and Nolan, John M. (2016) Quantification of zeaxanthin stereoisomers and lutein in trout flesh using chiral high-performance liquid chromatography-diode array detection. Journal of Food Composition and Analysis, 50. pp. 19-22. ISSN 0889-1575

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Abstract

In our previous work we identified the presence of meso-zeaxanthin [(3R,3'S)-zeaxanthin] in trout flesh and skin (Nolan et al., 2014), but were not able to quantify this carotenoid with the method used at that time. In the present study, we developed a protocol that allows for the quantification of lutein and the three stereoisomers of zeaxanthin [(3R,3'R)-zeaxanthin, meso-zeaxanthin and (3S,3'S)-zeaxanthin] in fish flesh. We tested this protocol in two species of farmed trout (Oncorhynchus mykiss and Salmo Trutta), and we detected and quantified these carotenoids. The concentrations of each carotenoid detected (ranging from 1.18 ± 0.68 ng g-1 flesh for meso-zeaxanthin to 38.72 ± 15.87 ng g-1 flesh for lutein) were highly comparable for the two fish species tested. In conclusion, we report, for the first time, the concentrations of zeaxanthin stereoisomers (including meso-zeaxanthin) and lutein in trout flesh. This work adds further to the knowledge on the presence of these carotenoids in the human food chain.

Item Type:	Article
Additional Information:	Funding Information: APC is a Howard Fellow and his research program is supported by the Howard Foundation (English Charity reg. number 285822) via a commercial grant from Iosa (Industrial Orgánica S.A., Monterrey, Nuevo León, Mexico), MacuHealth LLC™ (Birmingham, MI, USA) and Alliance Pharma plc (Chippenham, Wiltshire, UK); all of the above organisations have an interest in commercially available supplements containing the macular carotenoids. JMN and SB do consultancy work for nutraceutrical companies as directors of Nutrasight Consultancy Ltd. AH is an ‘honorary director’ of Howard Foundation Holdings Limited and Nutriproducts Ltd., which licence and supply nutraceutical ingredients. The sponsors had no involvement in study design, collection, analysis or interpretation of data, in the writing of the manuscript or in the decision to submit the article for publication. Publisher Copyright: © 2016.
Uncontrolled Keywords:	/dk/atira/pure/subjectarea/asjc/1100/1106
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Depositing User:	Admin SSL
Date Deposited:	19 Oct 2022 23:06
Last Modified:	24 Jun 2023 06:56
URI:	http://repository-testing.wit.ie/id/eprint/4047

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