Efficient species identification of pine marten (Martes martes) and red fox (Vulpes vulpes) scats using a 5′ nuclease real-time PCR assay

O'Reilly, Catherine and Statham, Mark and Mullins, Jacinta and Turner, Peter D. and O'Mahony, Declan (2008) Efficient species identification of pine marten (Martes martes) and red fox (Vulpes vulpes) scats using a 5′ nuclease real-time PCR assay. Conservation Genetics, 9 (3). pp. 735-738. ISSN 1566-0621

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Abstract

Monitoring wildlife species by DNA identification of samples collected non-invasively is an important tool in conservation management. DNA identification of species from faecal (scat) samples is problematic due to the small quantities and poor quality of the DNA isolated from such samples. This study demonstrates the use of real-time PCR technology in the identification of red fox (Vulpes vulpes) and pine marten (Martes martes). It is shown that real-time PCR can be used to identify fox and pine marten by either melting curve analysis (Tm determination) with SYBR Green 1 detection or by the use of species specific fluorogenic probes. The technique is shown to work efficiently with scat DNA.

Item Type:	Article
Uncontrolled Keywords:	/dk/atira/pure/subjectarea/asjc/1100/1105
Departments or Groups:
Depositing User:	Admin SSL
Date Deposited:	19 Oct 2022 23:06
Last Modified:	07 Jun 2023 18:44
URI:	http://repository-testing.wit.ie/id/eprint/4091

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