The cyanide hydratase enzyme of Fusarium lateritium also has nitrilase activity

Nolan, Linda M. and Harnedy, Padraigin A. and Turner, Peter and Hearne, Audrey B. and O’reilly, Catherine (2003) The cyanide hydratase enzyme of Fusarium lateritium also has nitrilase activity. FEMS Microbiology Letters, 221 (2). pp. 161-165. ISSN 0378-1097

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The filamentous fungus Fusarium lateritium produces cyanide hydratase when grown in the presence of cyanide. The cyanide hydratase protein produced at a high level in Escherichia coli shows a low but significant nitrilase activity with acetonitrile, propionitrile and benzonitrile. The nitrilase activity is sufficient for growth of the recombinant strain on acetonitrile, propionitrile or benzonitrile as the sole source of nitrogen. The recombinant enzyme shows highest nitrilase activity with benzonitrile. Site-directed mutagenesis of the F. lateritium cyanide hydratase gene indicates that mutations leading to a loss of cyanide hydratase activity also lead to a loss of nitrilase activity. This suggests that the active site for cyanide hydratase and nitrilase activity in the protein is the same. This is the first evidence of cyanide hydratase having nitrilase activity.

Item Type: Article
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/2400/2404
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Depositing User: Admin SSL
Date Deposited: 19 Oct 2022 23:07
Last Modified: 25 Jun 2023 02:45

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