The application of dispersive liquid–liquid microextraction in the analyses of the fatty acid profile in bovine milk in response to changes in body condition score

Quigley, Andrew and Connolly, Damian and Cummins, Wayne (2018) The application of dispersive liquid–liquid microextraction in the analyses of the fatty acid profile in bovine milk in response to changes in body condition score. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1073. pp. 130-135. ISSN 1570-0232

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Abstract

Dispersive liquid–liquid microextraction (DLLME) was used prior to gas chromatography flame ionization detection (GC-FID) for the extraction of five fatty acids from milk taken from cows with different body condition scores. Optimum extraction conditions were: 300 μL of chloroform (extraction solvent), and 1 mL methanol (dispersive solvent). The procedure was optimised using Design of Experiments (DoE). The analytes were separated on a GC capillary column containing a polyethylene glycol stationary phase (15 m × 0.53 mm × 1.2 μm). Enrichment factors were in the range of 8–15 and limit of detection (LOD) was 0.04 μg/mL. Calibration graphs showed good linearity with coefficients of determination higher than 0.994% and relative standard deviations lower than 7%. This method provided a simple and rapid derivatisation and extraction method for the determination of fatty acids in bovine milk. It showed that there was a significant difference in the palmitic acid content of milk from cows that had an optimum body condition score (10.85 mg/mL) compared to cows that had a high body condition score (5.73 mg/mL).

Item Type:	Article
Additional Information:	Funding Information: This study was funded by the Irish Research Council [grant number GOIPG/2015/3681 ]. Publisher Copyright: © 2017
Uncontrolled Keywords:	/dk/atira/pure/subjectarea/asjc/1600/1602
Departments or Groups:
Depositing User:	Admin SSL
Date Deposited:	19 Oct 2022 23:07
Last Modified:	07 Jun 2023 18:44
URI:	http://repository-testing.wit.ie/id/eprint/4163

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