Exploring the roles of and interactions among microbes in dry co-digestion of food waste and pig manure using high-throughput 16S rRNA gene amplicon sequencing 06 Biological Sciences 0605 Microbiology 09 Engineering 0907 Environmental Engineering

Jiang, Yan and Dennehy, Conor and Lawlor, Peadar G. and Hu, Zhenhu and McCabe, Matthew and Cormican, Paul and Zhan, Xinmin and Gardiner, Gillian E. (2019) Exploring the roles of and interactions among microbes in dry co-digestion of food waste and pig manure using high-throughput 16S rRNA gene amplicon sequencing 06 Biological Sciences 0605 Microbiology 09 Engineering 0907 Environmental Engineering. Biotechnology for Biofuels, 12 (1). ISSN 1754-6834

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Abstract

Background: With the increasing global population and increasing demand for food, the generation of food waste and animal manure increases. Anaerobic digestion is one of the best available technologies for food waste and pig manure management by producing methane-rich biogas. Dry co-digestion of food waste and pig manure can significantly reduce the reactor volume, capital cost, heating energy consumption and the cost of digestate liquid management. It is advantageous over mono-digestion of food waste or pig manure due to the balanced carbon/nitrogen ratio, high pH buffering capacity, and provision of trace elements. However, few studies have been carried out to study the roles of and interactions among microbes in dry anaerobic co-digestion systems. Therefore, this study aimed to assess the effects of different inocula (finished digestate and anaerobic sludge taken from wastewater treatment plants) and substrate compositions (food waste to pig manure ratios of 50:50 and 75:25 in terms of volatile solids) on the microbial community structure in food waste and pig manure dry co-digestion systems, and to examine the possible roles of the previously poorly described bacteria and the interactions among dry co-digestion-associated microbes. Results: The dry co-digestion experiment lasted for 120 days. The microbial profile during different anaerobic digestion stages was explored using high-throughput 16S rRNA gene amplicon sequencing. It was found that the inoculum factor was more significant in determining the microbial community structure than the substrate composition factor. Significant correlation was observed between the relative abundance of specific microbial taxa and digesters' physicochemical parameters. Hydrogenotrophic methanogens dominated in dry co-digestion systems. Conclusions: The possible roles of specific microbial taxa were explored by correlation analysis, which were consistent with the literature. Based on this, the anaerobic digestion-associated roles of 11 bacteria, which were previously poorly understood, were estimated here for the first time. The inoculum played a more important role in determining the microbial community structure than substrate composition in dry co-digestion systems. Hydrogenotrophic methanogenesis was a significant methane production pathway in dry co-digestion systems.

Item Type:	Article
Additional Information:	Funding Information: Funding for this study was provided by the Green Farm project supported by a Science Foundation Ireland Investigator Project Award (Ref: 12/IP/1519). Xinmin Zhan also wants to thank the support of Natural Science Foundation of China (Ref: 51728801). Publisher Copyright: © 2019 The Author(s).
Uncontrolled Keywords:	/dk/atira/pure/subjectarea/asjc/1300/1305
Departments or Groups:
Depositing User:	Admin SSL
Date Deposited:	19 Oct 2022 23:09
Last Modified:	13 Aug 2023 22:50
URI:	http://repository-testing.wit.ie/id/eprint/4359

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