Identification of novel serum microRNAs in age-related macular degeneration

Elshelmani, Hanan and Wride, Michael A. and Saad, Tahira and Rani, Sweta and Kelly, David J. and Keegan, David (2020) Identification of novel serum microRNAs in age-related macular degeneration. Translational Vision Science and Technology, 9 (4). pp. 1-13. ISSN 2164-2591

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Abstract

Purpose: To identify circulating microRNAs (miRNA) associated with age-related macular degeneration (AMD). Thus differentially expressed serum miRNA could be used as AMD biomarkers. Methods: This study involved total RNA isolation from sera from patients with atrophic AMD (n = 10), neovascular AMD (n = 10), and age-and sex-matched controls (n = 10). A total of 377 miRNAs were coanalyzed using array technologies, and differentially regulated miRNAs were determined. Extensive validation studies (n = 90) of serum from AMD patients and controls confirmed initial results. Total RNA isolation was carried out from sera from patients with atrophic AMD (n = 30), neovascular AMD (n = 30), and controls (n = 30). Fourteen miRNAs from the discovery dataset were coanalyzed using quantitative real-time polymerase chain reaction (qRT-PCR) to validate their presence. Results: Unsupervised hierarchical clustering indicated that AMD serum specimens have a different miRNA profile to healthy controls. We successfully identified and validated the differentially regulated miRNAs in serum from AMD patients versus controls. The biomarker potential of three miRNAs (miR-126, miR-19a, and miR-410) was confirmed by qRT-PCR, with significantly increased quantities in serum of AMD patients compared with healthy controls. Conclusions: Increased quantities of miR-126, miR-410, and miR-19a in serum from AMD patients indicate that these miRNAs could potentially serve as diagnostic AMD biomarkers. All three miRNAs significantly correlated with AMD pathogenesis. Translational Relevance: The discovery of new AMD miRNA may act as biomarkers in evaluating AMD diagnosis and prognosis.

Item Type:	Article
Additional Information:	Funding Information: Supported by the Irish Research Council for Science, Engineering, and Technology/EMBARK Initiative, the Mater Vision Institute, the Libyan Ministry of Higher Education and Scientific Research, and Fighting Blindness Ireland (FB14 SAA). Publisher Copyright: © 2020 The Authors.
Uncontrolled Keywords:	/dk/atira/pure/subjectarea/asjc/2200/2204
Departments or Groups:
Depositing User:	Admin SSL
Date Deposited:	19 Oct 2022 23:01
Last Modified:	13 Aug 2023 02:05
URI:	http://repository-testing.wit.ie/id/eprint/3647

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